Why would a comparison of the genomic and cDNA sequences ide

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helpmeplz
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Why would a comparison of the genomic and cDNA sequences ide

Post by helpmeplz » Wed Feb 04, 2009 10:13 am

Why would a comparison of the genomic and cDNA sequences identify the donor and acceptor splice sites?

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zami'87.
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Re: Why would a comparison of the genomic and cDNA sequences ide

Post by zami'87. » Sun Aug 23, 2009 5:18 am

genomic DNA..actually genes have exons+introns...cDNA you get from mRNA(thus it contains only exons).If you mix genomic and cDNA and heat them..they will denaturate..then you lower temperature and cDNA will hybridize with genomic DNA on matching parts-exons..rest are introns...so points where hybridization takes place are points where exons begin. hope I understood your question correctly
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