checking quality and quanitfying cDNA

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spin2
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checking quality and quanitfying cDNA

Post by spin2 » Mon Feb 02, 2009 2:01 pm

I have been having lots of troubles gettign robust PCR reactions to work from cDNAs that I have stored in the -20. I have checked the RNA quality by gel electrophoresis and get nice rRNA bands and no degredation. However, depending on the day some of my cDNA give me products and some do not. Is there a way to check the quantity and quality of cDNA? Can you spec it and get anything measurable? can you run a gel?

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MrMistery
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Post by MrMistery » Wed Feb 04, 2009 3:20 am

well i don't really deal with nucleic acids that much, but can't you just do the same electrophoresis-based test you did for the rRNA on the cDNA?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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