primers in reading frame

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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minabayardi
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primers in reading frame

Post by minabayardi » Wed Jan 28, 2009 9:56 pm

HI! ¡HOLA!

I HAVE A QUESTION ABOUT THE TOPIC. IF SOME ONE CAN HELP ME, PLEASE, I NEED IT. I AM DESIGNING PCR OLIGOS, AND IN THE FUTURE WE COULD USE THEM TO SCREENING A DNA LIBRARY. IS TOTALLY IMPORTANT THAT THE PRIMERS ARE IN READING FRAME? IF THE ANSWER IS YES, PLEASE TELL ME HOW TO DO IT.
THANKS.
I AM VERY SORRY BECAUSE OF MY BAD ENGLISH, PLEASE IMAGINE THAT IS NOT BAD AT ALL. SORRY.

MINA.

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canalon
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Post by canalon » Wed Jan 28, 2009 11:06 pm

No.

But you also want to stop posting all in capital letters. On the internet, it is similar to shouting. And that is rude.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

minabayardi
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Re: primers in reading frame

Post by minabayardi » Thu Jan 29, 2009 5:57 pm

Well, I did not know... I do not wanted be rude in any posible way. I have to apologize for do not know this rule.

Mina

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