immunoblotting and Elisa assay

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forensicbio87
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immunoblotting and Elisa assay

Post by forensicbio87 » Thu Jan 22, 2009 12:21 pm

everything i have read so far has only told me what they are but i want to know how accurate are both of these methods?

forensicbio87
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Post by forensicbio87 » Thu Jan 22, 2009 12:23 pm

i mean for identifying and seperating protiens

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GreenDog
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Post by GreenDog » Thu Jan 22, 2009 5:55 pm

Very, Very, Very accurate.
If you have a good antibody you'll see exactly your protein.
"When In Danger Or In Doubt Run In Circles Scream And Shout"
Lawrence J. Peter

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Sepals
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Post by Sepals » Fri Jan 23, 2009 3:03 pm

You can not seperate proteins with ELISA. Immunoblotting can be tricky, that's my experience.
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