Document on CHO

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Fruitfly
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Document on CHO

Post by Fruitfly » Fri Jan 16, 2009 2:55 am

I'm doing with CHO K1 and CHO dhfr-, but I don't have much information about them. I want to know their origin, biochemistry and physiology. If someone know webs or aticles, etc...about them, would you mind sharing with me ?
Thank you very much :D
PS: wishing to hear from you soon

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jonmoulton
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Re: Document on CHO

Post by jonmoulton » Fri Jan 16, 2009 3:31 pm


einstein
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Post by einstein » Sat Jan 17, 2009 8:19 pm

what is CHO

Fruitfly
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Re: Document on CHO

Post by Fruitfly » Mon Jan 19, 2009 1:03 am

Thanks to jonmuolton
To einstein: CHO is chinese hamster ovary, a commercial cell line for producing pharmaceutical proteins
By the way, I have a question: There are many kind of CHO (eg. CHO K1, CHo dhfr-) available commercially, but they all lack of biosynthetic pathway of proline. Why isn't CHO which can grow in medium no proline used ?

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jonmoulton
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Re: Document on CHO

Post by jonmoulton » Mon Jan 19, 2009 5:52 pm

I don't know, but I am willing to make a wild guess.

Perhaps there is enough available proline in a Chinese hamster ovary that the wild-type cells are missing a functional gene for proline biosynthesis.

Like I said, this is a guess. Does anyone have a more solid idea about this?

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Re: Document on CHO

Post by Fruitfly » Tue Jan 20, 2009 3:52 am

Oh, I think your idea may be possibly right. I don't know whether CHO really is popular cell line used on the wold beacause I've found it very much but what I gained is little than I expect. Can you see me any lab doing with this cell ?

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