Anti-fungals or not?

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pinkypig
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Anti-fungals or not?

Post by pinkypig » Wed Jan 14, 2009 1:44 pm

ok i am very confussed about this...

A researcher at my university when doing cell culutre experiments vows not to use anti-fungal drugs in the medium but all other reasearchers do and i realy do not understand why he doesn't use them?

Does anybody know why he might not use them?

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biohazard
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Post by biohazard » Wed Jan 14, 2009 2:55 pm

I have personally never used anti-fungal drugs in my cultures; good aseptic technique is well enough to keep your cultures clean. Unlike anti-microbial drugs, anti-fungal ones target eukaryotic cells, and thus the chances of side effects are much bigger with anti-fungal drugs. Could you give me an example of what kind of drugs people over there use?

I don't know anyone around here who'd use anti-fungal drugs in their cultures, but then again, it probably depends on many factors, like what cells you use, what's the aim of your experiment and so forth.

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MrMistery
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Post by MrMistery » Thu Jan 15, 2009 3:35 pm

I've never heard of anti-fungal drugs in cell cultures either. Just an antibiotic in the growth medium and a good sterile technique were always enough to prevent infections...
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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