Aseptic conditions!

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderators: Leonid, amiradm, BioTeam

Post Reply
pinkypig
Garter
Garter
Posts: 31
Joined: Tue Jan 29, 2008 8:48 pm

Aseptic conditions!

Post by pinkypig » Tue Jan 13, 2009 11:01 pm

why is it important in cell culture to work under aseptic conditions when the growth medium already contains antibiotics?

Any ideas?

blcr11
Viper
Viper
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Re: Aseptic conditions!

Post by blcr11 » Wed Jan 14, 2009 6:57 am

Neither yeast (spores are everywhere) nor mycoplasma (you are probably carrying some on your hands right now) don't care squat about antibiotics.

User avatar
MichaelXY
King Cobra
King Cobra
Posts: 885
Joined: Fri Jun 15, 2007 7:03 pm
Location: San Diego, Ca

Post by MichaelXY » Wed Jan 14, 2009 7:07 am

In what units are squats given.

User avatar
biohazard
King Cobra
King Cobra
Posts: 776
Joined: Sun Apr 08, 2007 6:45 pm

Post by biohazard » Wed Jan 14, 2009 11:46 am

Also, mycoplasma contamination is often extremily difficult to notice, especially during the early phase (so called cryptic contamination), during which you may easily spread it further or think that the results you get from your experiments are valid even though they're compromised by mycoplasma. Antibiotics often slow down the growth of mycoplasma (but don't kill it), so before you notice something is wrong, it's everywhere.

Yeast contamination, luckily, is much more easy to spot, so at least you know what cultures are affected and can ditch them.

pinkypig
Garter
Garter
Posts: 31
Joined: Tue Jan 29, 2008 8:48 pm

Re: Aseptic conditions!

Post by pinkypig » Thu Jan 15, 2009 8:17 pm

BUMP

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Fri Jan 16, 2009 5:26 am

why do you need to bump? seems pretty covered
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest