mrna in E coli

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crazy99
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mrna in E coli

Post by crazy99 » Sat Nov 29, 2008 2:56 am

in a piece of hemoglobin beta chain,how will u look to see if an mRNA exists in E coli.

what am not able to understand is that am i supposed to search for mrna in e coli 's hemoglobin beta chain.am confused how to go about this question.

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MrMistery
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Post by MrMistery » Sat Nov 29, 2008 4:20 am

did you clone the beta-hemoglobin gene in E.coli?
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jonmoulton
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Post by jonmoulton » Mon Dec 01, 2008 4:51 pm

It sounds like you want to determine whether an mRNA is being expressed from a beta-globin gene in E. coli. One approach is to look for a beta-globin sequence in the total mRNA expressed by the bacterium. Here are two techniques that could be useful:

http://en.wikipedia.org/wiki/RT-PCR

http://en.wikipedia.org/wiki/Northern_blot

MrMistery asked whether the beta-hemoglobin gene was cloned into E. coli. If so, that can simplify the problem. If the gene is inserted into a plasmid vector in the E. coli, you could do a plasmid prep and so produce a less complex population of nucleic acid to probe or sequence.

http://en.wikipedia.org/wiki/Plasmid

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