Inhibition of RNA/DNA/protein synthesis

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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RuNkLi
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Inhibition of RNA/DNA/protein synthesis

Post by RuNkLi » Thu Nov 13, 2008 9:07 am

hi,

I want to ask if there is a way to detect inhibition of macromolecules in bacteria by antibiotics with an non radioactive method.
normally we use tritium-labelled leucine, thymidine, uridine.

I know that these radioactive precursors are very sensitive, but is there a non radioactive method?

Can someone help me in this case?

THX

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MrMistery
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Post by MrMistery » Thu Nov 13, 2008 4:01 pm

maybe you could tag them with small florochromes. No ideea if you can find one that is small enough to allow your amino acid to still be incorporporated into a nucleotide chain, and that might also present problems on tRNA loading. OK, so it's not a great idea, but if you don't want to radioactivity its the only thing I can think of
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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