cell fractionation

Discussion of all aspects of cellular structure, physiology and communication.

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gorgi
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cell fractionation

Post by gorgi » Wed Nov 12, 2008 8:28 pm

how could the contents of a pellet be examined to confirm that it contains mainly mitochondria??
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blcr11
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Re: cell fractionation

Post by blcr11 » Thu Nov 13, 2008 9:58 am

Electron microscopy? That's kind of a pain and I don't know what kind of shape they'll be in after isolation. You could do enzyme assays to confirm (Krebs cycle enzymes, maybe) and, to some extent rule out contamination (some glycolytic enzyme or DNase maybe? not sure what people usually use.).

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MrMistery
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Post by MrMistery » Thu Nov 13, 2008 3:58 pm

A good bet might be an MTT assay. But i think you will need a standard curve, which you probably would need to make for yourself - which might be just going back to your problem.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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