Too much DTT

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TurdFerguson
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Too much DTT

Post by TurdFerguson » Fri Oct 31, 2008 10:46 pm

I set up a reaction recently in which I was examining a cytosolic chaperone's activity. It is possible I may have had 10 mM DTT in the reaction buffer, rather than the suggested 1 mM DTT. Is it possible this could interfere with my protein's activity?

-Undergrad dude

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