RNA precipitation after extraction: role of LiCl ?

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faerstd
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RNA precipitation after extraction: role of LiCl ?

Post by faerstd » Mon Oct 20, 2008 10:30 am

Hi,

I have to write my protocol after a course in Molecular Biology, where in one lecture we extracted the RNA from the cells. After doing so, we precipitated the RNA with Ethanol and Lithium chloride for 3 hours.

Now my question: what's the role of LiCl in this precipitation? Wouldn't the RNA be precipitated just with Ethanol?
Can I say LiCl binds to other substances left after the extraction, like DNA ?

have to understand why we used LiCl ..

any ideas? please help.

thanks in advance. faerstd

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Post by MrMistery » Mon Oct 20, 2008 7:19 pm

"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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Re: RNA precipitation after extraction: role of LiCl ?

Post by faerstd » Mon Oct 20, 2008 7:43 pm

i'm so stupid.. i searched google indeed, but didn't read that !!!! :lol:

so this licl specially gets large dehydrated RNA molecules make aggregates so that the precipitation works better

thanks a lot.. :oops:

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