electrophoration protocol

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jasmina
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electrophoration protocol

Post by jasmina » Thu Sep 25, 2008 8:57 am

I have to do transfection experiment these day, but this is the first time for me to do electrophoration ,so i don't have complete electrophoration protocol. Is there anyone who can offer me an electrophoration protocol?my cell is Raw 264.7.THANK YOU VERY MUCH!

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jonmoulton
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Re: electrophoration protocol

Post by jonmoulton » Thu Sep 25, 2008 6:49 pm

Hi Jasmina,

It would help to know what you are delivering, what cells or tissues you are delivering it into and what sort of electroporation apparatus you will use.

I doubt this is what you have in mind, but here's something to start with: delivery of Morpholino oligos into cultured cells by nucleofection with an Amaxa electroporator.
http://www.gene-tools.com/files/Amaxa_M ... 008-04.pdf

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