Conservation of samples/DNA during an expedition

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Katanaji
Garter
Garter
Posts: 1
Joined: Thu Sep 18, 2008 9:16 am

Conservation of samples/DNA during an expedition

Post by Katanaji » Thu Sep 18, 2008 9:36 am

Hi!

I am preparing my first expedition. I will be going to Africa to study mycorrhizae.

To do so, I will be taking cores around trees.

I probably wont have much of a lab available, and it will be several weeks before I am back to England or the USA.

What would you advise?
I was told that I could maybe sort my roots and fungi on site and use CTAB buffer, but the method seems quite complicated and apparently requires a lot of sample material, so I am not sure if it can be applied to microscopic roots...

Any idea is welcome!

Thanks a lot :)

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Fri Sep 19, 2008 2:04 am

You could probably resuspend your samples in tubes containing a reagent that would denature proteins like Guanidium isothiocyanate (GITC) or Guanidium Hydrochloride and freeze/refrigerate yoour samples until you go back to your lab.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest