Counting plant mitochondria

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Cromatida
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Counting plant mitochondria

Post by Cromatida » Wed Sep 17, 2008 2:41 pm

Hello!

Does anyone now an easy method of counting extracted plant mitochondria? I would need en exact number of the mitochondria that I extracted. I don't have conditions for Flow cytometry and I'm also not interested in proteins to use Bradford assay.
I'm thinking about plase contrast microscopy. Could anyone give me a protocol about the treatment that I have to use for this (dyes, steps). Is there a chemical that could make them bigger? So they would be more easy to count?

Thank you in advance!

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