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About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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elizabethw
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Post by elizabethw » Mon Sep 08, 2008 12:26 pm

Hmm I have this question in my lab book and I think I know the answer, but I am probably wrong. :)

When measuring bacteria size, why is it better to measure live bacteria than stained bacteria?

I keep thinking that it might be because they're distorted when heat stained.

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alextemplet
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Post by alextemplet » Mon Sep 08, 2008 6:51 pm

The heat used to stain them can evaporate the water that makes up about 70% or so of the bacterial cytoplasm, thereby possibly distorting the cell's size.
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