cell cryopreservation in -80
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cell cryopreservation in -80
hi, there will have any consequences on my peripheral blood mononuclear cells if i transfert them from nitrogen liquid to -80.
please help me because liquid nitrogen is very expensive for my laboratory
thanks for helps
please help me because liquid nitrogen is very expensive for my laboratory
thanks for helps
Our lab has the view that PBMCs start to deteriorate after around one month of storage in -80 C, therefore you need liquid nitrogen or similar for proper long-term storage of your cells.
However, my personal experience is that the cells stay viable longer, I have succesfully thawed at least 2-3 month old cells (frozen in RPMI + 20% AB serum & 10% DMSO), and my colleague claims that he has stored PBMCs in -80 C for more than six months without problems.
But if you want to play it safe, then I'm afraid you need colder conditions than -80 C.
However, my personal experience is that the cells stay viable longer, I have succesfully thawed at least 2-3 month old cells (frozen in RPMI + 20% AB serum & 10% DMSO), and my colleague claims that he has stored PBMCs in -80 C for more than six months without problems.
But if you want to play it safe, then I'm afraid you need colder conditions than -80 C.
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