cell cryopreservation in -80

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cell cryopreservation in -80

Post by tregis6 » Mon Aug 18, 2008 7:38 am

hi, there will have any consequences on my peripheral blood mononuclear cells if i transfert them from nitrogen liquid to -80.
please help me because liquid nitrogen is very expensive for my laboratory :cry: :cry: :cry:
thanks for helps

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King Cobra
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Post by biohazard » Mon Aug 18, 2008 12:18 pm

Our lab has the view that PBMCs start to deteriorate after around one month of storage in -80 C, therefore you need liquid nitrogen or similar for proper long-term storage of your cells.

However, my personal experience is that the cells stay viable longer, I have succesfully thawed at least 2-3 month old cells (frozen in RPMI + 20% AB serum & 10% DMSO), and my colleague claims that he has stored PBMCs in -80 C for more than six months without problems.

But if you want to play it safe, then I'm afraid you need colder conditions than -80 C.

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