cell cultur medium

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tregis6
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cell cultur medium

Post by tregis6 » Wed Jul 30, 2008 4:53 pm

dear all,
i am in ruptur of minimum essential medium, now is it possible to prepar my complete medium without D-MEM?.With what can i replace the MEM which play the same role?
for 50ml of complet medium i use : 43,5 ml of RPMI + 5 ml of FBS + 0,5 ml MEM + 0,5 ml of L-GLU + 0,5 mL of STREP-PENICYLINE.
I prepar the complete medium to thaw PBMCs frozen in nitrogen liquid and to do three hours of cell cultur whit PMA + IONOMICIN + BREFELDIN A.
thanks for helps

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