Discussion of all aspects of cellular structure, physiology and communication.
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I have cloned something with IRES-EGFP under CMV promoter to generate transgenic mouse. When I checked transfection efficiency of the clone in vitro, I got only 20% cells expressing GFP. Is it necessary to get high expression of the gene in vitro to make transgenic mice? In other words, will it not be possible to generate transgenic mice with this clone?
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