checking transfection efficiency before transgenic mice gene

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mnl
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checking transfection efficiency before transgenic mice gene

Post by mnl » Wed Jul 16, 2008 9:46 am

I have cloned something with IRES-EGFP under CMV promoter to generate transgenic mouse. When I checked transfection efficiency of the clone in vitro, I got only 20% cells expressing GFP. Is it necessary to get high expression of the gene in vitro to make transgenic mice? In other words, will it not be possible to generate transgenic mice with this clone?

Istria
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Post by Istria » Sat Jul 26, 2008 8:47 pm

These are completely different systems and so you should have no problem.

As long as your clone generates your protein and GFP correctly, you are GOOD.

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