Spleen analysis

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Spleen analysis

Post by DoctorStein » Sat Jul 05, 2008 11:40 am


I am preparing such a labwork of Hematology, and the chapter is RBC. I list some parameters to analyse RBC in rat body, including: RBC count, hemoglobin concentration, and hematocrit. I need more parameters and I am thinking about spleen as blood reservoir organ. However, I have no idea what parameter I can use of the spleen. I list about splenic morphometry (mass, volume), splenic RBC count, splenic hematocrit, and red pulp investigation (I have no idea what I will do for red pulp!).

I remember there's someone from Hematology lab here but I can't recall who is who. Hopefully he (I 'm sure the one is he, a male) will reply this one :)


I found this abstract.... sounds interesting (the one I highlight), unfortunately I don't have the fulltext :(

Influence of the spleen on blood volume and haematocrit in the brush-tailed possum (Trichosurus vulpecula)

TJ Dawson and MJS Denny


The blood volume of T. vulpecula was measured and the influence of the spleen on the circulating blood volume investigated. The circulating blood volume of "normal" restrained animals was 57.4 ± 3.19 ml, the plasma volume being 31.2 ± 1.93 ml, and the red blood cell volume 26.2 ± 2.08 ml per kilogram body weight. These values tended to be lower than those of eutherian mammals and it is suggested that this might be associated with a possible lower metabolic rate. The spleen was found to have a significant function as a blood reservoir. Measurement of volume of circulating red blood cells after injections of adrenaline (to cause splenic emptying) and chlorpromazine (to achieve maximum filling of the spleen) showed that the splenic reserve of erythrocytes was approximately 11.0 ml/kg body weight.

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