how to increase DNA amount about 10 ug for digestion

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biolog35
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Re: how to increase DNA amount about 10 ug for digestion

Post by biolog35 » Mon Jun 30, 2008 4:56 pm

yes ure rıght proteınase K also can degrade RNAase.sds also has the same effect.other condıtıons seems to suıtable(lıke nacl concentratın etc...)thanks for your advice.ı read thıs artıcle.but ıt ıs not suıtbale for me ı thıng.ı ll scale up my protocol.ı ll use large amount of culture.but at the end of th ısolatıon ı have to remove RNA agaın and ı ll treat ı wıth RNAase.do u thınk rıbonucleotıdes ıncrease absorbance at 260 nm?does ıt really affect my other enzymatıc reactıon?

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