How to find out if a gene was present in more than 1 locus?

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angelyn1985
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How to find out if a gene was present in more than 1 locus?

Post by angelyn1985 » Wed Jun 18, 2008 10:26 pm

Hi! I am wondering if someone has the answer to my question!! I can't find the information i need in my textbooks.

I know that by using suitable primers in PCR, we can find out if a certain gene is present in a DNA sample or in the total DNA of an organism. But after proving the presence of the gene, what other experiment(s) can be done to find out if the gene was present in more than 1 location in the genome?

I will appreciate any kind of clue! thanks!!

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mith
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Post by mith » Wed Jun 18, 2008 11:06 pm

cut your sequences up
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

Cat
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Post by Cat » Wed Jun 18, 2008 11:20 pm

1. Isolate total DNA.
2. Digest large amount with the enzyme (6 cutter preferred) that does NOT cut your gene overnight.
3. Run a long gel (5-6HR at 30V).
4. Perform southern blot.
5. Analyze results: 1 band = 1 locus, etc...

Hope this helps.

angelyn1985
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Re: How to find out if a gene was present in more than 1 locus?

Post by angelyn1985 » Thu Jun 19, 2008 1:11 am

THanks alot!!

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