Binding of peptide to bacteria

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RuNkLi
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Binding of peptide to bacteria

Post by RuNkLi » Wed Jun 18, 2008 9:40 am

I have wt and mutant bacteria lacking membrane ancored lipid.

The peptide binds to a certain lipid on the bacterial surface.

How can i smartly show, that this peptide binds less to the mutant when compared with the wildtype?

Which method is here the optimal resolution?

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MrMistery
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Post by MrMistery » Mon Jun 23, 2008 6:35 pm

I don't quite understand your question, but from what i get you are trying to show that your mutant lacks a special lipid which binds to a certain peptide. the obvious choice is make the peptide bind a fluorochrome and then measure the intensity.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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