Binding of peptide to bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 11
Joined: Tue Jan 31, 2006 10:58 am
Location: Germany

Binding of peptide to bacteria

Post by RuNkLi » Wed Jun 18, 2008 9:40 am

I have wt and mutant bacteria lacking membrane ancored lipid.

The peptide binds to a certain lipid on the bacterial surface.

How can i smartly show, that this peptide binds less to the mutant when compared with the wildtype?

Which method is here the optimal resolution?

User avatar
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)

Post by MrMistery » Mon Jun 23, 2008 6:35 pm

I don't quite understand your question, but from what i get you are trying to show that your mutant lacks a special lipid which binds to a certain peptide. the obvious choice is make the peptide bind a fluorochrome and then measure the intensity.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Post Reply

Who is online

Users browsing this forum: Baidu [Spider] and 2 guests