uH2A Ab source

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
vickynini
Garter
Garter
Posts: 1
Joined: Mon Jun 16, 2008 8:29 pm

uH2A Ab source

Post by vickynini » Mon Jun 16, 2008 8:31 pm

Millipore/Upstate recently discontinued their uH2A antibody(cat# 05-678). I was wondering if anyone knows any other sources of uH2A antibody. Thanks. :D

Chribu
Garter
Garter
Posts: 3
Joined: Thu Jul 10, 2008 3:26 pm

Re: uH2A Ab source

Post by Chribu » Thu Jul 10, 2008 4:22 pm

I am in dire need of this antibody too. I have already contacted the person who originally established the uH2A antibody producing hybridoma cell line. He told me that the clone probably died (!?) and that his lab ran out of the antibody. Any information about alternative sources is highly appreciated.

So far, It looks like H2A pulldown and probing for ubiquitin is the only way.

DanWebster
Garter
Garter
Posts: 3
Joined: Fri Jul 25, 2008 11:56 pm

Re: uH2A Ab source

Post by DanWebster » Mon Jul 28, 2008 5:11 pm

Hi Guys,

I too have been struggling to find this antibody. Has anyone called Millipore to see if they plan on re-instating anti-uH2A anytime soon? If not, I may be able to make one, but it would take a while. Please post updates if you find anything.

Thanks!

Chribu
Garter
Garter
Posts: 3
Joined: Thu Jul 10, 2008 3:26 pm

Re: uH2A Ab source

Post by Chribu » Wed Aug 13, 2008 11:13 pm

I called Millipore and also the person that initially established the uH2A antibody producing hybridoma. He told me that the clone died and that is why Millipore discontinued this antibody.

Yes, please make a new uH2A antibody. :)

DanWebster
Garter
Garter
Posts: 3
Joined: Fri Jul 25, 2008 11:56 pm

Re: uH2A Ab source

Post by DanWebster » Wed Aug 20, 2008 6:23 pm

I am confused. I called Millipore and they said they had 20 units of the antibody left. I am going to try and order it today, and will update as to whether I can get it or not

DanWebster
Garter
Garter
Posts: 3
Joined: Fri Jul 25, 2008 11:56 pm

Re: uH2A Ab source

Post by DanWebster » Wed Aug 20, 2008 10:27 pm

Hey Guys,

I don't know who you talked to at Millipore, but my uH2A antibody (05-678) is coming tomorrow. Give them a call and get some!

Best,
DW

Chribu
Garter
Garter
Posts: 3
Joined: Thu Jul 10, 2008 3:26 pm

Post by Chribu » Mon Aug 25, 2008 9:02 pm

You are right Dan! I just called Millipore and now the antibody is available again. However, they do only have 3 units left at the moment.

Hurry up guys!

Post Reply

Who is online

Users browsing this forum: Google [Bot] and 7 guests