lysosomal enzymes don't interfere with protein

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ss_kalve
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lysosomal enzymes don't interfere with protein

Post by ss_kalve » Thu Jun 05, 2008 12:32 pm

Hi, can anyone tell in the process of protein extraction we crush the sample in mortar pastel. By this process lysosomal enzymes should come out and interfere with protein.so wat to do for that?

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MrMistery
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Post by MrMistery » Thu Jun 05, 2008 3:51 pm

Lysosomal enzymes are only active at a low pH, so you shouldn't have any problems with that in theory. In practice, I would keep the samples on ice if possible.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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