Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Hi, can anyone tell in the process of protein extraction we crush the sample in mortar pastel. By this process lysosomal enzymes should come out and interfere with protein.so wat to do for that?
- Inland Taipan
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Lysosomal enzymes are only active at a low pH, so you shouldn't have any problems with that in theory. In practice, I would keep the samples on ice if possible.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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