Specific specific OR SCAR

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
rkmkbk
Garter
Garter
Posts: 1
Joined: Fri May 23, 2008 9:34 am

Specific specific OR SCAR

Post by rkmkbk » Fri May 23, 2008 9:38 am

I have a RAPD primer which produces a polymorphic band (of around 540 bp) for 5 species of a same genus. Now I want to convert to Species Specific Primer/SCAR. My expectation is one primer to differentiate all 5 species. It means I have to synthesis a single primer which will produce bands of different bp in electrophoresis gel. Is is possible? If so, please let me get the step by step procedure.

blcr11
Viper
Viper
Posts: 672
Joined: Fri Mar 30, 2007 4:23 am

Re: Specific specific OR SCAR

Post by blcr11 » Fri May 23, 2008 12:19 pm

Try here and references therein:
http://crop.scijournals.org/cgi/reprint/43/1/345

Post Reply

Who is online

Users browsing this forum: Google Adsense [Bot] and 8 guests