Making SOC solution

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
samkh918
Garter
Garter
Posts: 20
Joined: Thu May 01, 2008 9:27 pm

Making SOC solution

Post by samkh918 » Tue May 13, 2008 4:52 am

Hello,

Could you please let me know how to prepare SOC solution from LB, MgCl2, MgSO4, and glucose?
I have the following recipe but I'm not sure if that's correct:

800 ul LB
4 ul MgCl2 (1M)
4 ul MgSO4 (1M)
16 ul glucose (1M)


Thanks for your help.

snowcapk
Garter
Garter
Posts: 37
Joined: Fri Oct 26, 2007 2:01 am

Post by snowcapk » Tue May 13, 2008 8:10 am

My suggestions:
    Make the LB and adjust the pH to 7. I recommend adding Tris at pH 7 to act as a buffer, maintaining the correct pH in the SOC medium as bacteria grow.
    Autoclave the LB and wait for it to cool. (Mmm, caramel!)
    Add the glucose, MgCl2, and MgSO4. (I would go for final concentrations of 10mM MgCl2, 10mM MgSO4, and 20mM glucose. This is about double the MgCl2 and MgSO4 concentrations you've given, but I doubt it matters.)
    Filter-sterilize (w/ 0.2um filter according to Qiagen.)

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests