Looking for: Protocol to Culture HESCs without Feeder

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biolearner
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Looking for: Protocol to Culture HESCs without Feeder

Post by biolearner » Mon May 12, 2008 8:31 pm

I have been doing experiments with HESCs, H1 and H9 (from national stem cell bank). Right now, I am co-culturing the cell lines with feeder cell line MEF (from ATCC).

I wonder if anyone has the experience to culture H1 and/or H9 without using feeder layer. You are very appreciated if you can let me know the protocol.

Thank you.

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