vehicle treated apoptosis

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biology_06er
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vehicle treated apoptosis

Post by biology_06er » Sat May 10, 2008 2:35 pm

Hi there,

Just wondering, we had Neuro2A cells treated with water and had to count the # or apoptotic cells...technically there should not be any...buuut we had like 5ish..what are sum reasons for this..just spontaneous?

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b_06er

snowcapk
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Post by snowcapk » Sat May 10, 2008 8:35 pm

Just plain water? Maybe osmotic shock.

biology_06er
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Post by biology_06er » Sat May 10, 2008 10:25 pm

yep...well we had to make incubations with buffer and wash it out then add again etc with a step of adding cresyl violet..so is osmotic shock still the case?

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MrMistery
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Post by MrMistery » Mon May 12, 2008 4:46 pm

I'm confused. Did you use distilled water or a buffer solution?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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