sequence of steps for PCR, etc

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upinder1955
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sequence of steps for PCR, etc

Post by upinder1955 » Thu May 08, 2008 5:33 am

What is the sequence of steps carried out for
1. PCR
2. cloning a gene
3. DNA finger-printing
4. taking out pure DNA from a cell
Please reply. It is urgent.

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canalon
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Post by canalon » Thu May 08, 2008 2:57 pm

1-see for example: http://en.wikipedia.org/wiki/PCR
2- look up for the rest by yourself
3-come back with questions that show you have thought about your homework
4-receive help

Yes spoon feeding homework answers is not the site policy.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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mith
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Post by mith » Thu May 08, 2008 4:42 pm

It's urgent though. lol, better delegate it to the internets.
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pguo
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Re: sequence of steps for PCR, etc

Post by pguo » Thu May 08, 2008 5:31 pm

I think you are doing something right now, so it's urgent to know the protocols for your experiments. am I right?

You can find PCR and its related protocols over here http://www.lab-manual.com/lm_013.htm

There is another place you can find DNA, RNA, protein and their related, such as molecular cloning background and protocols. http://www.e-biotek.com/ebiotek_304.htm

In addition, you can easy find some ebooks and handbooks at above links. Let me know if I can help further.

Good luck.

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