glucose transporters

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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canalon
Inland Taipan
Inland Taipan
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Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Sun May 18, 2008 2:09 am

Mr Mistery is right:
-RTqPCR if you have a few genes. Depending on the machines you can multiplex 3 to 5 genes (and that would include a control housekeeping gene to normalize for variations in RT efficiency). The positive side is that you can work with a truckload of samples (say 40 in a 96 wells plates if you include duplicates, controls and normalization curve) everyday (a common program would run in 2 to 3 hours, so you can easilly run 2 to 3 plates a day)
-plenty of genes, then it becomes worthy to move to microarrays. But it is usually harder to work with a truckload of samples, since the experiment is time consuming and demands a lot of work. Someone I know was considering 6 samples a day a ggod result. But 100's of gene per sample. There is a trade-off. Unless you can afford robots to do the work.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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