Spore counting

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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pinkypig
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Spore counting

Post by pinkypig » Mon May 05, 2008 11:57 am

What is the procedure for visualising spores to perform a total count on them? Taking into account that spores are difficult to stain.

pinkypig
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Post by pinkypig » Tue May 06, 2008 10:58 am

can someone help me with this plzzz!!!ineed to know asap!

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Post by pinkypig » Tue May 06, 2008 6:18 pm

why is nobody repling to this post??is it because no one knows the answer??

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canalon
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Post by canalon » Tue May 06, 2008 7:21 pm

For fungus I know they stain with calcofluor white and then count with epifluorescence microscope.
Looking at he litterature other methods: are:
-Heat shock to reactivate the spore and plating and suitable medium
-Haemocytometer, but I guess that must be for eukariotyc parasites
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

pinkypig
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Post by pinkypig » Tue May 06, 2008 7:55 pm

No i need to know about staining spores, i have heard somthing about methyl orange and malachite green but not entirely sure,so if anyone knows anymore info about these methods let me know plz!

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Post by Cat » Mon May 12, 2008 11:40 pm


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