buffer

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jojo90
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buffer

Post by jojo90 » Sun Apr 27, 2008 3:00 am

i've encountered a qns that i dunno how to do
hope u guys can help me out thx.

the qns:
a student was told to dilute Tris-HCL buffer from 1M to 10mM before using it for a biological assay. Instead of doing that, he used the 1M solution directly in his assay, citing that a higher concentration of the buffer would providebetter buffering capacity.State if you agree with him and why.

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mith
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Post by mith » Sun Apr 27, 2008 4:51 am

The question is asking two things I think--

1. Is the student's explanation correct? i.e. does 1M buffer provide better buffering?

2. If true, is this a good idea?
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

jojo90
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Re: buffer

Post by jojo90 » Sun Apr 27, 2008 5:31 am

haha thxz but i still dunno how to do

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mith
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Post by mith » Sun Apr 27, 2008 2:41 pm

You can start by looking up or reading up on how a buffer works.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

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