mannose extraction with methanol/chloroform

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spinkster
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mannose extraction with methanol/chloroform

Post by spinkster » Thu Apr 24, 2008 9:39 am

Hi,

ive been looking in my biochem books for why mannose can be extracted with methanol/chloroform in glycoprotein synthesis.

I dont really get it, does the methanol/chloroform attach to a dolicol which is attached to two phosphate groups which is then attached to mannose?

any help on this would be great (as i am probably not understanding it correctly)

cheers

Matt

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MrMistery
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Post by MrMistery » Thu Apr 24, 2008 10:22 am

yes,i think you have it exactly on target. Glycoprotein synthesis occurs by attaching sugars evolved from dolichol phosphate. Dolichol, of course, is a lipid, and can be extracted by using methanol/chloroform.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

spinkster
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Re: mannose extraction with methanol/chloroform

Post by spinkster » Thu Apr 24, 2008 2:48 pm

Also do you know if dolicol has any other roles in the cell.
I know it has another function as a GPI anchor but is anyone aware of any others?

cheers

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