Question about cloning using SCNT...

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Wingman
Garter
Garter
Posts: 1
Joined: Wed Apr 23, 2008 7:46 pm

Question about cloning using SCNT...

Post by Wingman » Wed Apr 23, 2008 7:51 pm

I've just been reading about cloning from somatic cells and in particular the example of Dolly the sheep, and there's just one thing I'm not sure about...

Why were two donor sheep used to clone Dolly? i.e. Why were the cytoplasmic donor and the nuclear donor not the same sheep? Surely if the somatic nucleus and the female germ cell had come from the same sheep the resulting lamb would be a 'better' clone since its mitochondrial genome would also be the same as that of the donor sheep...
Top
User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:
Contact mith

Post by mith » Wed Apr 23, 2008 10:28 pm

Because then you can prove you can clone males.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
Top
User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Thu Apr 24, 2008 3:04 pm

Males or female, I would say the point is with that you can really easily prove that the clone is really the produce of the experiment and not a freakish accident. If you have the correct markers it is easy to show that the mt an nuclear genomes are coming from the 2 different individuals, ergo that your cloning worked.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Top
Post Reply

Return to “Genetics”

Who is online

Users browsing this forum: No registered users and 3 guests