Identifying an Unkown bacteria.

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
nadialindsay
Garter
Garter
Posts: 1
Joined: Fri Apr 18, 2008 2:32 pm

Identifying an Unkown bacteria.

Post by nadialindsay » Fri Apr 18, 2008 2:59 pm

Hi I need help identifying an unknown bacteria.

According to my gram stain I have a gram negative bacteria that are bacillus shaped. However the other test that I have performed suggest that I may have a Gram positive bacteria. The test are as follow:

Catalases Results are negative
Amalyse results are negative
Bacteria does not digest Casine
TSI: Bacterial growth on agar slant the agar was turned yellow/yellow with no bubbles.
SCA: Bacteria did not catablolize citrate
EMB: There was no bacterial growth
MSA: Bacteria turned the media bright yellow.
Pseud Agar F: Clear color colonies on media
VRB: No colony growth on media
MacConky Agar: No colony growth on media.

I need to identify at least the family name. Any help is appreciated.

User avatar
mkwaje
Coral
Coral
Posts: 146
Joined: Mon Jul 03, 2006 2:48 am
Location: Philippines

Post by mkwaje » Mon May 19, 2008 1:35 pm

I could hazard a guess on this one but then I should be the one to get a grade. You can identify this correctly, with a bit of work, using Bergey's Manual of Determinative Bacteriology. I'm pretty sure your library has this. Its much easier to get your answers from the net from other people but its really more satisfactory if you could just spend a little more time with the Bergey's bible. Good luck!

User avatar
Sepals
Coral
Coral
Posts: 364
Joined: Sat Dec 31, 2005 12:45 am
Location: Manchester

Post by Sepals » Sat May 24, 2008 1:17 pm

I advise Cowan and Steel's manual for the identification of medical bacteria. That's what I use.
Image

Xubu
Garter
Garter
Posts: 2
Joined: Fri Jul 18, 2008 2:06 pm

Re: Identifying an Unkown bacteria.

Post by Xubu » Fri Jul 18, 2008 2:51 pm

why dont you use the Molecular Biology technique in this case? The most accurate method to identify your unknown bacteria is to sequencing its 16S rDNA, i think.

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Wed Jul 23, 2008 5:39 pm

That's rRNA, not DNA. And that is really expensive, you know..
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Re:

Post by canalon » Wed Jul 23, 2008 7:56 pm

MrMistery wrote:That's rRNA, not DNA. And that is really expensive, you know..


No sequencing of the DNA coding for the 16S rRNA. One PCR, and depending of the fragment length 1 or 2 sequencing reaction. Less than 30$ if you do not need to order the primers specifically for that (I assume externalization of sequencing and in lab PCR). So quite cheap in fact.

But I suspect this would problably not help the original poster who was probably trying to figure out the solution to his lab...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Thu Jul 24, 2008 10:23 am

That might be cheap where you live, not everywhere :P
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Nad
Garter
Garter
Posts: 13
Joined: Mon Jul 14, 2008 1:29 pm

Re:

Post by Nad » Thu Jul 24, 2008 1:57 pm

MrMistery wrote:That might be cheap where you live, not everywhere :P


Romania suxs. If a book sells at Amazon with 140 USD, the idiots from Romania inist to sell it with 170 EUR. And so and so and so....

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Thu Jul 24, 2008 4:17 pm

Nad, you're from Romania?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Nad
Garter
Garter
Posts: 13
Joined: Mon Jul 14, 2008 1:29 pm

Post by Nad » Thu Jul 24, 2008 7:59 pm

Yes, MrMistery, we share this honor of living in Romania :P

Post Reply

Who is online

Users browsing this forum: No registered users and 8 guests