Filter particles out of insect cell lysate

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
nexus2006
Garter
Garter
Posts: 4
Joined: Tue Nov 20, 2007 9:43 am

Filter particles out of insect cell lysate

Post by nexus2006 » Tue Apr 08, 2008 5:09 pm

Hello,

i have an insect cell lysate with some large protein particles, which are ca. 20um x 3um, i like to separate from the rest debris swimming around. I thought about filtering cause centrifuging steps bring them down together.
Thank you very much for any ideas!
Regards,

Rudi

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Tue Apr 08, 2008 11:02 pm

You can do column chromatography(filtering)
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

Post Reply

Who is online

Users browsing this forum: No registered users and 12 guests