How to purify single-stranded cccDNA

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houlinlin
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How to purify single-stranded cccDNA

Post by houlinlin » Tue Apr 01, 2008 8:46 am

Hi,
does anyone know how to make single-strand cccDNA from double-strand plasmid? And how to purify?
Thanks a lot. :D

Cat
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Re: How to purify single-stranded cccDNA

Post by Cat » Tue Apr 01, 2008 6:33 pm

Run it on TAE agarose gel. See picture here:

http://humgen.wustl.edu/hdk_lab_manual/ ... mid10.html

I am not sure, though, if you can keep it in that form.

You might find these useful:

http://aem.asm.org/cgi/reprint/56/6/1725
http://www.jbc.org/cgi/reprint/264/2/1051.pdf

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