study of herbal antiseptics on ecoli

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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amangaur
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study of herbal antiseptics on ecoli

Post by amangaur » Sat Mar 22, 2008 1:35 pm

hi i am working on the project(effects of herbal antiseptics on ecoli)
can anyone give me a protocol of the same?????????????
i am just interested in finding out the estimated number of ecoli b4 & after d exp.

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canalon
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Post by canalon » Sun Mar 23, 2008 2:12 am

In short:
- Dilute (in 10 fold increments)
- Plate (a known volume, on a rich non selective medium, if possible in triplicates)
- Count (the colonies, on plate with between 30 and 300 colonies, and with the average and the dilution factor you can compare the number of colonies per inoculum in your different conditions)
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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