Protein expression

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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roniadam
Death Adder
Death Adder
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Protein expression

Post by roniadam » Thu Mar 20, 2008 1:51 pm

HI
Can anyone tell me why is the frame of the inserted DNA important in bacterial expression system while it is not important for mammalian and baculovirus system?

Cat
King Cobra
King Cobra
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Post by Cat » Thu Mar 20, 2008 2:45 pm

I am not sure I understand your question. DNA inserted where – into a transformation vector or host genome? Also, what is the insert that it has to be in frame? ORF without ATG? You see in most bacterial expression systems your plasmid transformation vector is usually transcribed directly…

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