Shine dalgarno sequence

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georgina009
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Shine dalgarno sequence

Post by georgina009 » Wed Mar 12, 2008 12:07 am

In a E. coli mRna strand what would happen if the shine dalgarno sequence was removed? Would mRNA still be transcribed from the gene? Would protein still be translated?

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MrMistery
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Post by MrMistery » Wed Mar 12, 2008 12:15 pm

do you know the role of the Shine-Dalgarno sequence?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

georgina009
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Post by georgina009 » Wed Mar 12, 2008 11:15 pm

I know the role but that dosent answer my question

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mith
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Post by mith » Thu Mar 13, 2008 2:13 am

If the role is to make something happen, then removal would cause it to not happen?

Consequently, if it has no role in making something happen, then removal would have no effect.
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Cat
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Post by Cat » Thu Mar 13, 2008 2:21 am

Very devious and cryptic reply, Mith… I like it a lot!

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Post by blcr11 » Thu Mar 13, 2008 1:53 pm

Think of it as a way of controlling how often a baseball pitching machine throws a ball. If there is a Shine-Dalgarno gear in place, the machine throws 100 balls a minute. If you take out the gear, it only throws 10 balls a minute. If each ball represents 1 polypeptide chain, compare the results of running the machine for one minute with and then without its Shine-Dalgarno gear in place.

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mith
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Post by mith » Thu Mar 13, 2008 2:31 pm

really, Cat, I thought it was more logic than bio
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