DIG system

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
biology_06er
Coral
Coral
Posts: 260
Joined: Tue Mar 21, 2006 9:33 am

DIG system

Post by biology_06er » Tue Mar 04, 2008 12:25 pm

Hi there,

Just wondering it says on our lab sheet that once the procedure for the DIG system is complete (putting them in various buffers etc etc) and then it says how many dots have appeared..so im guessing ill see that when the time comes...its the next part im confused about...it says record the concentration of each visible dot..!!! How do I do that?? finding the conc. from a dot?? :( ..I don't get it...

Any help..MUCH appreciated

Thanks,
biology_06er

User avatar
jonmoulton
Viper
Viper
Posts: 442
Joined: Fri Feb 15, 2008 5:38 pm
Location: Philomath, Oregon, USA

Re: DIG system

Post by jonmoulton » Tue Mar 04, 2008 8:42 pm

Sounds like a densitometry problem, but I wouldn't be terribly confident of quantitative reproducibility with a chromogenic system like most digoxigenin-based assays. The usual quick-and-dirty approach is to scan the blot and measure the amount of blackness of each dot/band, using software like NIH Image or Photoshop. This is better for comparisons between signals on a single blot than for absolute quantitation.

Post Reply

Who is online

Users browsing this forum: abulkalamazad017, Google Adsense [Bot] and 11 guests