To whom working with DNA sequencing

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roniadam
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To whom working with DNA sequencing

Post by roniadam » Wed Feb 27, 2008 11:32 pm

1. Would a primer extension reaction in DNA sequencing work using an RNA polymerase? any one can explain this point please?
2. When the products of a DNA sequencing reaction are separated by electrophoresis, why is it normally only possible to read about 500 to 800 bases, yet the sequencing reaction continues for thousands of bases?

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canalon
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Post by canalon » Thu Feb 28, 2008 3:02 pm

1- look up initiation of the reaction for both enzymes. besides think about RNA stability.
2- think about the termination mechanism and the relation between probability of synthesis of a fragment of increasing length and the intensity of the signal.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

roniadam
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Post by roniadam » Fri Feb 29, 2008 10:50 am

Still cannot find the answers

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canalon
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Post by canalon » Sat Mar 01, 2008 2:30 am

read this, and notably paragraph 3.1:

http://en.wikipedia.org/wiki/Rna_Polymerase
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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