Competent cells

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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mmustafa
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Competent cells

Post by mmustafa » Wed Feb 27, 2008 1:33 am

Can someone tell me where I can get DH5alpha stock from to make my own competent cells?

Cat
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Post by Cat » Wed Feb 27, 2008 3:03 am

Your best choice is to ask microbiology and biotechnology professors. Making competent cells should be a part of biotech lab curriculum.
Good Luck.

mmustafa
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Post by mmustafa » Wed Feb 27, 2008 3:09 pm

Can't I buy it from somewhere?

blcr11
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Re: Competent cells

Post by blcr11 » Wed Feb 27, 2008 4:16 pm

Almost any company that supplies cloning kits will probably sell you some DH5a cells. New England Biolabs I know sells them, so probably do Novagen, Invitrogen, and Stratagene, and possibly Qiagen. You can do better than DH5a. And you can also buy competent cells rather than make them yourself. The Giga format (NovaBlue Giga, for example) from Novagen works well for me.

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