Genetics as it applies to evolution, molecular biology, and medical aspects.
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- Posts: 1
- Joined: Wed Feb 20, 2008 2:30 pm
hey der ppl can ne1 ...pls pls help me by tellin me excatly wat is ligation mediated PCR.... pls help me as soon as possible as i hav my xamz on my head ....
- King Cobra
- Posts: 635
- Joined: Thu Feb 14, 2008 7:40 pm
Basically, if you know only part of your sequence and have only one primer, you will need to ligate a linker or adaptor (for the second primer) in order to isolate unknown sequence. It is greatly used in isolation of the insertion flanking sequences in mutants.
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