wat is ligation mediated PCR??

Genetics as it applies to evolution, molecular biology, and medical aspects.

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yashashri2287
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wat is ligation mediated PCR??

Post by yashashri2287 » Tue Feb 26, 2008 10:36 am

hey der ppl can ne1 ...pls pls help me by tellin me excatly wat is ligation mediated PCR.... pls help me as soon as possible as i hav my xamz on my head ....
:(

Cat
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Post by Cat » Tue Feb 26, 2008 9:56 pm

Basically, if you know only part of your sequence and have only one primer, you will need to ligate a linker or adaptor (for the second primer) in order to isolate unknown sequence. It is greatly used in isolation of the insertion flanking sequences in mutants.

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