microbiology

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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imran sajid
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microbiology

Post by imran sajid » Tue Jun 14, 2005 8:07 am

Microorganisms restrict the growth of others by secreting specific antimicrobial compounds what is so called antagonism. i m working on the discovery of new antimicrobial cmpounds or antibiotics would some one suggest me how the antimicrobial activity of a microbe can be checked agains other on the plate.stooped answering is not required as i got on my previous question.

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b_d_41501
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Post by b_d_41501 » Tue Jun 14, 2005 3:41 pm

Didn't realize Kirby-Bauer or the E-Test was a stupid answer? :? But I do think that this is what you are looking for. You compare the MIC and MBC of the antibiotics that you are using on the organisms.
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canalon
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Post by canalon » Tue Jun 14, 2005 5:18 pm

E-test and Kirby bauer can work only with purified antibiotics (and only fot the one available in E-test format, but you can buy empty disc that yu load with whatever you need for the Kirby bauer), hence it is probably not a good solution.

I never did that but you can probaly try to have the 2 bugs on one plate and look for inhibition at the boundary between the 2 cultures. Or try adding a culture supernatant to a 'Kirby-Bauer' like disk and see if those discs inhibits bacterial growth.

Good luck

Patrick

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b_d_41501
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Post by b_d_41501 » Tue Jun 14, 2005 5:31 pm

Yes, that will work. We did that in my Genetics lab as well.
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kmpennebaker
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another idea?

Post by kmpennebaker » Fri Jul 22, 2005 8:50 am

I think its called burkholder agar assay where you pour a plate using soft agar and imbed test bug in the agar and plate different cells on top. Looks for soluble agents.

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