Help --GFP

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jia
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Help --GFP

Post by jia » Thu Jan 24, 2008 7:37 pm

Reccently I got a Tie-2- GFP ES cell line and I should differentiat these ES cells into blood vessels and then check them under the confocal miroscope. But the problem is that I can not see any of the green gluorescence in several days of differentiation when the blood vessel morphology have been shown after stained with CD31 antibody . The tie-2 is expressed on the endothelial cells during angiogenersis . So I do not know what's the problem is . By the way the ES cells given by the other lab have been sucessfully used in the differentiation of the blood vessels . So could anyone tell me what happened to my cells, what I gona do

jia
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Re: Help --GFP

Post by jia » Mon Jan 28, 2008 3:30 pm

do any one could tell me how many possibilites there are for not generating furorescent by the GFP

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