Plasmid Isolation From Vibrio

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kofi
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Plasmid Isolation From Vibrio

Post by kofi » Tue Jan 08, 2008 12:56 pm

Hello. How can I isolate plasmid DNA from Vibrio strains??

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canalon
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Post by canalon » Tue Jan 08, 2008 2:04 pm

Many DNA purification kits will collect plasmid DNA and avoid genomic DNA, provided you have a good lysis protocol. Just like described here: http://www.msk.or.kr/jsp/view_old_journ ... erSeq=2543
Patrick

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any proof. (Ashley Montague)

kofi
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Re: Plasmid Isolation From Vibrio

Post by kofi » Wed Jan 09, 2008 6:41 pm

Dear Canalon

Yes, I've tried these protocols, but they didn't give me results. This means that my strain doesn't have a plasmid? It appears to carry Kanamycin resistance. Can this characteristic be a chromosomic one?

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canalon
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Post by canalon » Wed Jan 09, 2008 7:41 pm

This cannot be excluded, and if you look at D. Mazel work on Vibrio spp. superintegrons, it becomes even more likely. But having a control, i.e. a Vibrio strain with a known plasmid, would allow you to check your protocol.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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