coomassie staining

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piefke
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coomassie staining

Post by piefke » Mon Dec 10, 2007 10:18 am

hi
I will run some protein gels today. Now I have one question: I will stain them with coomassie. Is it possible to destain them over night? or will the protein bands dissappear ?? :?

blcr11
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Post by blcr11 » Mon Dec 10, 2007 1:56 pm

You should be OK overnight in the standard destain of 30% MeOH, 10% HOAc. It is possible to completely destain a coomassie-stained gel, but you usually up the MeOH to 40-50% and go for at least 24 hours. You can leave the gel in stain overnight and destain in the morning, or transfer a partially destained gel to water for the overnight, and continue with destain in the morning. All of that works.

piefke
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Post by piefke » Mon Dec 10, 2007 4:15 pm

Thank you!! :wink:

ss_kalve
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Post by ss_kalve » Sat Dec 15, 2007 5:17 am

U wash the gel with 45-50 C hot water.it'll really work.gv time for wash abt 20-30min each.After 4-5 wash it'll destain easily.[/code]

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