ok, how much water to 10nmol to get 100µM?

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seidili
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ok, how much water to 10nmol to get 100µM?

Post by seidili » Thu Dec 06, 2007 7:32 pm

Hey

I finally see my problem with my primers. I received them nondiluted. So the amount is given as 10nmol. How much water do I have to add to get the concentration of 100µM?

Thnx

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canalon
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Post by canalon » Thu Dec 06, 2007 8:17 pm

10 nmol in powder? or liquid. In the later case you just dilute using the famous CiVi=CfVf (C for concentration, initial or final, V is for volume). If in powder you have 10e-8 mol.
100µM = 10e-4 mol/L = 10e-7 mol/ml = 10e-8 mol/100µl
Patrick

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seidili
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Post by seidili » Thu Dec 06, 2007 8:27 pm

it's in powder. so simply add 100µl TE?

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